Epagri is carrying out a project aimed at the induction of mutants for resistance to banana diseases by irradiation of in vitro shoots of the cultivar SCS451 Catarina. The first step was the determination of sensitivity to gamma rays using doses of 0; 12.5; 25; 37.5 and 50 Gy. As it is known if in a shoot tip after treatment with any mutagen the mutation may be induced in M1V1, however, there...
The demand for food is set to increase due to a growing population while on the contrary, climate change is emerging to be the biggest threat to agricultural production as more lands are becoming marginalized. To cope with the increasing demand for food and other plant-based products, the need to diversify and intensify crop production could not be more emphasized. Significant progress in the...
Wad Ahmed is a Sorghum variety popular among farmers in Sudan, except that it matures slightly late and is (too) tall making it prone to yield losses caused by terminal drought and lodging. Mutagenesis and breeding work was undertaken to obtain early-maturing and semi-dwarf mutants and we are developing molecular markers to facilitate use of these new traits in breeding programs. Seed bags of...
Pepper is one of the most important vegetable crops in the Solanaceae family. Although the complete genome sequence has been reported and genetic studies have been performed for many important traits in pepper, a broader range of genetic resources for breeding and functional genomics studies are still required. Therefore, we developed mutant populations using radiation and an optimized a...
Aluminium is the third most abundant element in the Earth’s crust. In acidic soils it solubilizes to Al3+ ions, that are phytotoxic and cause root growth inhibition. Around 50% of arable lands are acidic. Barley is one of the crops most sensitive to Al it is a good model for studying Aluminium response. The common mechanism of Aluminium tolerance is related to excretion of organic acids. Here...
Kala Jirga, a local non-basmati aromatic rice cultivar (landrace) grown in Kolhapur district, Maharashtra, India possesses a good aroma quality, it has low yield potential and a very good potential for domestic market. 500 seeds bulks of Kala Jirga cultivar were subjected separately to mutagenic treatments with gamma rays (100, 200 and 300Gy), Ethyl methanesulphonate (EMS) 0.8, 1.0 and 1.2%...
Improvements to massively parallel sequencing have allowed the routine recovery of natural and induced variations in genomic DNA sequence. Thus, such approaches can be considered for routine application in plant mutation breeding projects. The need for high sequence coverage to accurately recover single nucleotide variants and small insertions and deletions limits the applicability of whole...
The occurrence of low temperatures is a common stress in rice cultivation in temperate regions, making tolerance to low temperatures a desirable trait in rice genotypes grown in Southern Brazil. These low temperatures hinder germination, crop establishment and grain yield. This study aimed to characterize and identify through the TILLING technique, rice families tolerant to low temperature...
Cavendish bananas are sterile, parthenocarpic and an obligate vegetatively propagated crop. This makes them particularly susceptible to diseases including Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (Foc) tropical race four (Foc TR4). Foc TR4 has been identified in nine countries and its recent spread is threatening global banana production. Previous experiments showed that when...
We have developed a unique technology for mutation induction of plants using energetic ion beams at the RI Beam Factory (RIBF) of RIKEN. Ion beams effectively induce mutations at relatively low doses without severely inhibiting growth. The irradiation treatment can be given to various plant materials and mutation can be induced in a short time between seconds and a few minutes. We have...
Coffee, a perennial tropical crop, can be grown from seeds or cloned plants in the form of cuttings, grafts or tissue cultured plants. Arabica coffee is most commonly grown from seeds while canephora is mostly grown vegetatively from cuttings and other propagules. Improving coffee through conventional breeding is seriously limited by the availability of genetic variation. Mutation breeding...
The effects of climate change have had an unfavourable impact on food production. Drought and high temperatures affect the production and supply of food to the population in Cuba. The development of new varieties tolerant to drought is an alternative that will be used to minimize the effects of climate change on food production and the induction of mutation has demonstrated its potential in...
Crop monocultures and the disappearance of small-farms is having a negative impact on the genetic diversity used in global agriculture. Enhancing agrobiodiversity plays a key role in adapting to a changing climate and feeding future generations. Expanding genetic diversity through induced mutagenesis is highly successful with thousands of improved varieties released worldwide. Advanced...
Striga is an obligate hemi-parasitic weed species that attacks roots of major cereal crops such as sorghum, millet, maize and rice. In severe situations of Striga infestation, losses in crop yield may reach up to 90-100%. Forty percent of arable land in sub-Saharan Africa is infested with Striga with annual losses estimated up to 14 billion US$. For subsistence farmers, growing resistant...
Crop improvement program through radiation induced mutation breeding was initiated nine decades ago. India has made significant progress in the field of mutation breeding in different crop species. Rice is a staple food for Indian population with nearly 70% population deriving daily dietary needs from rice. With changing consumer preferences, global climate change, changing pest dynamics and...
Lentil is the most widely preferable pulse crop, especially in Asia. It is predominantly grown under rain-fed condition. Drought is one of the main abiotic stresses that limit the plant growth and productivity in Bangladesh. A robust screening protocol is crucial for successful mutation breeding for drought tolerance. A pre-field screening method was optimized at the PBGL using polyethylene...
Legume plants (18,000 legume species exist) possess a complex root-developmental pathway, leading to nitrogen-fixing nodulation. Rhizobium bacteria induce cortical cell proliferation, then invade concurrently the newly developed tissue to differentiate into nitrogen-fixing bacteroids. This nodule development is of global significance for agriculture and ecology. We have used a variety of...
The Institute of Genetics “Ewald A. Favret” (IGEAF) INTA, has a long tradition in Plant Mutation Research that began in the year 1949. The IGEAF has made important contributions about the effects of different physical and chemical mutagens and mutator genes on barley and described novel uses of barley and wheat mutants in basic research and breeding. Besides, it has done a pioneering work...
Fusarium verticillioides is associated with maize wherever it is grown. The fungus can reduce yield and grain quality, and this is exacerbated by the production of toxic secondary metabolites, called fumonisins. Plant resistance is the best means to control F. verticillioides, but such resistance is not readily available in existing maize cultivars. In this study, gamma irradiation was used to...
TILLING (Targeting Induced Local Lesions IN Genomes) was first described in 2000 as a reverse genetics tool that combines classical mutagenesis and high-throughput mutation screening. In this presentation the current status of TILLING platforms in different species will be summarized. The lecture will focus on latest achievements of TILLING in functional genomics and in practical plant...
Tomato (Solanum lycopersicum L.) is an important economic crop worldwide. In Mauritius, three types of tomatoes are grown: cooking, salad and cherry tomatoes. The salad and cherry tomatoes are grown under hydroponic systems whereas the most widely consumed tomato, the cooking type, is cultivated in open fields and thus is subject to adverse climatic conditions. Tomato with increased tolerance...