Since 18 of December 2019 conferences.iaea.org uses Nucleus credentials. Visit our help pages for information on how to Register and Sign-in using Nucleus.
27-31 August 2018
IAEA, Vienna
Europe/Vienna timezone

Development of a Functional Marker for Marker-Assisted Selection of ‘Orange Lemma’ Mutants to Improve Feed Quality in Barley

Not scheduled
15m
IAEA, Vienna

IAEA, Vienna

Poster

Speaker

Ivan Ingelbrecht (IAEA)

Description

Barley is a major cereal crop for feed consumed by a wide range of animals including cows, pigs and poultry as green forage, hay, silage or grain. Multiple mutations exist in barley that can be exploited to develop specific forage barley varieties. Barley ‘orange lemma’ (rob1) mutants show similar reduced lignin content to mutants in maize or poplar. A range of barley ‘orange lemma’ varieties exist either as natural or induced mutants. Most genes in the lignin biosynthesis pathway have been well characterized opening perspectives to develop a functional marker for orange lemma using a candidate gene approach. Here we describe the molecular and genetic characterization of orange lemma in different spring barley accessions. Candidate causative mutations underlying the orange lemma phenotype were identified. An allele-specific assay was developed for rob1 in the ‘Bowman’ genetic background. Genetic and phenotypic analysis of the F1 derived from the rob1 mutant and its parental line showed that orange lemma is a monofactorial recessive trait. Using a segregating F2 population, trait and marker linkage could be demonstrated. Experiments have been initiated to validate the marker assay in additional barley genetic backgrounds to enable marker-assisted backcrossing of rob1 for feed barley improvement.

Primary authors

Ivan Ingelbrecht (IAEA) Bernhard Hofinger (Plant Breeding and Genetics Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency) Eda Akgun (Plant Breeding and Genetics Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture) Mirta Matijevic (Plant Breeding and Genetics Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture) Adel Ali (Plant Breeding and Genetics Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, IAEA Laboratories Seibersdorf) Joanna Jankowicz-Cieslak (Plant Breeding and Genetics Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture) Luka Jarc (Plant Breeding and Genetics Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture) Ljupcho Jankuloski (Plant Breeding and Genetics Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, IAEA) Abdelbagi Mukhtar Ali Ghanim (Plant Breeding and Genetics Labratory, Joint FAO/IAEA Division of Nuclear Applicatication in Food and Agriculture) Heinrich Grausgruber (Dept. Crop Sciences)

Presentation Materials

There are no materials yet.