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27-31 August 2018
IAEA, Vienna
Europe/Vienna timezone


Not scheduled
IAEA, Vienna

IAEA, Vienna

Poster Mutation breeding for adaptation to climate change in seed propagated crops


Dr Shatha Yousif (Ministry of Science & Technology, Baghdad-Iraq)


Hydroponic culture was performed to investigate the physiological responses of three mutant lines ‎with their wild-type parent under drought stress. Mutants; Noor 5, ‎Noor6 and Noor7 along with cv. Al Noor (parent) were subjected to 2 levels (0 and 10%) of polyethylene ‎glycol (PEG-6000). Growth at the seedling stage was evaluated. Differential Display Reverse ‎Trancriptase- DDRT was applied to study the genetic markers related with drought tolerance using 9 random primers (OPA-1, OPA-5, OPA-11, OPC-4,‎‏ ‏OPC-8, OPF-12, OPH16, OPO11 and ‎OPR7). The mutants along with cv. Al Noor were also planted under rainfed conditions. Drought tolerance indices (Stress Tolerance Index, Mean Productivity, Geometric Mean Productivity, Stress Tolerance ‎and Stress Susceptibility Index) were calculated. Correlations between stress indices under the ‎hydroponics experiment and grain yield under rainfed conditions were investigated. ‎ Water stress reduced root length and fresh weight in all genotypes, but increased ‎shoot length, fresh and dry weight in mutants Noor 5 and Noor 6. The results revealed that PEG treatment had positive affects in seedling shoot length, shoot fresh weight, ‎root dry weight in the mutant genotypes, but not cv. Al Noor. All primers succeeded to amplify DNA ‎fragments. The result of field experiment revealed that Noor6 surpassed all genotypes in the number of ‎tillers m-1 and grain yield. Grain yield under water stress had positive and significant ‎correlations with major stress indices: shoot and root length, shoot and root fresh weight, shoot dry ‎weight and malic enzyme under hydroponic experiment Based on these data, it can be concluded that seedling growth parameters could be used as selection ‎criteria for drought tolerance in wheat. DDRT-PCR could differentiate the genotypes and ‎maybe used in developing markers related to drought tolerance.‎
Country or International Organization Ministry of Science & Technology, Baghdad-Iraq

Primary author

Dr Shatha Yousif (Ministry of Science & Technology, Baghdad-Iraq)


Mrs Maha Taeen (Ministry of Science & Technology, Baghdad-Iraq) Mrs Nora Abid (Ministry of Science & Technology, Baghdad-Iraq) Mrs Tagreed AbdulJabar (Ministry of Science & Technology, Baghdad-Iraq)

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