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27-31 August 2018
IAEA, Vienna
Europe/Vienna timezone

EVALUATION OF WHEAT AND BARLEY GERMPLASM STRESS RESPONSE BY NUCLEAR, OMICS AND PHYSIOLOGICAL APPROACHES

Not scheduled
15m
IAEA, Vienna

IAEA, Vienna

Poster New challenges and technologies in plant genomics and breeding

Speaker

Mr Lubomir Stoilov (Professor Dr)

Description

Breeding priority worldwide is the development of cereals adapted to stress factors like extreme temperatures, salinity and drought, limiting the growth, productivity and distribution of agricultural crops. Knowledge of the mechanisms governing stress response is a prerequisite for development of advanced breeding practices and new varieties. National IAEA project Bul5/014 for technical cooperation is aimed to highlight the adaptive potential and enhance the involvement of wheat and barley in breeding programmes directed towards more sustainable, environmentally friendly and cost-effective agriculture. Complex stress response to radiation, temperature and fungal diseases of representative wheat and barley genotypes from the national seed genebank was evaluated using molecular, biochemical and physiological approaches. Gene expression profiling by Real-time PCR, SNP-based genotyping, gene cloning and characterization, PCR–mediated screening of DNA repair activities, HRM and expression analyses of stress-related proteins was performed. Genotype characterization was complemented by estimation of physiological and biophysical parameters like photosynthetic activity, electrolyte leakage and malondialdehyde content, nutrient use efficiency and protease activity. Research was focused on improvement of wheat for disease resistance, radiation-induced barley stress response, nutrient use efficiency in wheat and characterization of abiotic stress response of wheat and barley through DNA fingerprinting and functional analyses of stress-related genes as well as physiological and biochemical studies under stress conditions. Advanced knowledge on the protective capacity and adaptability of wheat and barley genotypes, based on the molecular mechanisms underlying their response to abiotic stress and radiation was obtained. Transcriptional profiling of radiation-responsive genes revealed some features of the genetic control of DNA repair in barley. Complex molecular, biochemical and physiological screening of wheat and barley stress tolerance supports further development of effective breeding strategies towards alleviation of the harmful impact of biotic and abiotic stress factors on the productive capacity of cereals.
Country or International Organization Bulgaria

Primary author

Prof. Elena Todorovska (Agrobioinstitute, Bulgarian Agricultural Academy)

Co-authors

Prof. Atanas Atanassov (Joint Genomic Center, Sofia State University) Dr Georgi Bonchev (Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences) Dr Gergana Mihailova (Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences) Dr Goritza Rakleova (Joint Genomic Center, Sofia State University) Dr Irina Boycheva (Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences) Prof. Katya Georgieva (Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences) Mr Lubomir Stoilov (Professor Dr) Dr Lyudmila Simova-Stoilova (Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences) Dr Rossitza Rodeva (Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences) Dr Stefan Tsonev (Agrobioinstitute, Bulgarian Agricultural Academy) Dr Svetlana Misheva (Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences) Dr Tanya Karceva (Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences) Prof. Valya Vassileva (Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences) Dr Vasilissa Manova (Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences) Dr Zlatina Uhr (Institute of Plant Genetic Resources, Agricultural Academy) Zornitsa Stoyanova (Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences)

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