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27-31 August 2018
IAEA, Vienna
Europe/Vienna timezone

CREATING NEW MUTANTS OF CHICKPEA RESISTANT TO ASCOCHYTA RABIEI USING GAMMA RADIATION.

Not scheduled
15m
IAEA, Vienna

IAEA, Vienna

Poster Mutation breeding for adaptation to climate change in seed propagated crops

Speaker

Dr Yassine MABROUK (Centre National des Scinces et Technologies Nucleaires)

Description

Chickpea (Cicer arietinum L.) is one of the most popular vegetables in many regions of the world. Chickpea is an important pulse crop and a major source of protein in human diet. In Tunisia, because of biotic and abiotic constraints, the cultivated area and production is unstable and is decreasing. The presence of fungi including Ascochyta blight, Fusarium wilt, black collar and root rot, dry root rot, Phytophthora root rot and Pythium damping-off in some chickpea growing areas are considered limiting factors to the expansion of the crop. Due to ineffective control methods and the lack of resistant varieties, induced mutation is used as an alternative for the improvement of chickpea for resistance to Ascochyta blight. This study was conducted to determine the lethal dose of 50% of chickpea seeds (LD50) to determine the optimum mutation induction dose and select chickpea mutant lines resistant to Ascochyta rabiei. Irradiation of chickpea seeds with a range gamma ray doses allowed to determine the LD50 (150 Gy). Genetic variability among M2 plants derived by mutagenesis was verified by the ISSR technique. Among 34 studied mutant lines we selected three mutants resistant to Ascochyta rabiei in M2 and M3 generations.
Country or International Organization Tunisia

Primary author

Dr Yassine MABROUK (Centre National des Scinces et Technologies Nucleaires)

Co-authors

Dr Kamel Charaabi (LR Biotechnology and Nuclear Technologies, National Center for Nuclear Sciences and Technology (CNSTN). Sidi Thabet Biotechpole, 2020 Sidi Thabet, Ariana, Tunisia.) Prof. Martina Rickauer (Université de Toulouse, Laboratoire d’Ecologie Fonctionnelle et Environnement UMR 5245 CNRS-INP-UPS, ENSAT, 18 Chemin de Borde Rouge, F-31326 Castanet-Tolosan, France.) Dr mahiout djamel ((3) Laboratoire de Protection des cultures, Faculté des Sciences de la Vie et de la Nature, Université de Mostaganem, Algérie.) Prof. omrane belhadj ((2)Laboratoire de Biochimie et de Technobiologie, Faculté des Sciences de Tunis, Université de Tunis El-Manar 2092 Tunis, Tunisie.)

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