FAO/IAEA International Symposium on Plant Mutation Breeding and Biotechnology

THE INDUCTION OF POLYPLOIDS IN ‘IRIS SARI SCHOTT EX BAKER’ VIA IN VITRO TECHNIQUES

Not scheduled

15m

IAEA, Vienna

Poster Mutation breeding for ornamental and vegetatively propagated crops

Speaker

Dr SELAY DOGAN (REPUBLIC OF TURKEY MINISTRY OF FOOD, AGRICULTURE AND LIVESTOCK)

Description

Iris sari SCHOTT ex BAKER is an endemic plant which has a natural distribution area in Turkey. The plant, which has attractive flowers, is also known as “Ana kurtkulağı, Bahar çiçeği”. Tetraploids were induced successfully from in vitro plantlets of I. sari by treating micro-bulbs with colchicine. The colchicine doses tested were: 0.1 and 0.5 % during 2, 4 and 6 hours for each dose. Ploidy levels could be easily determined by flow cytometry. From a total of 45 surviving plantlets, 3 tetraploids were detected. The most efficient condition for inducing tetraploids seemed to be the treatment with 0.1 % colchicine for 4 hours. Comparison of the survival rate of the controls and tetraploid plants in vitro, showed significant differences. Additionally, the induced tetraploids in I. sari also presented larger stomata and decrease in stomata density, compared to control plantlets.