Speaker
Dr
Ayse SEN
(Department of Biology, Faculty of Science, Istanbul University, 34134, Vezneciler-Fatih, Istanbul, Turkey)
Description
Increasing aridity of semi-arid regions and limited water resources have led to a crucial necessity for improving crop resistance to drought. Induced mutations play a significant role in adaptation to stress conditions, mutants are valuable germplasm resources in crop improvement programs. Molecular association mapping technique through linkage disequilibrium is one of the other approaches successfully used in breeding programs. As reported in publications, it can be effectively used to tag genomic regions involved in drought stress tolerance. In view of the above, during the presented work, we have focused on investigating population structure, linkage disequilibrium, and association mapping between pairs of SSR marker loci in F2 segregated population derived from reciprocal backcrossed mutant wheat germplasms under drought stress and non-stressed conditions. To associate markers with drought tolerance at vegetative stage, we examined over the 170 polymorphic SSR markers that were nearly evenly distributed on the 42 wheat chromosomes. A total of 100 segregated individuals grown under normal water supply and stressed conditions were observed for morphological and physiological parameters variations. Phenotypic and genotypic data were associated using TASSEL 2.1 to find associated markers with agronomic traits. The distribution of phenotypic variance was measured as varying between 7% and 17%. Three SSR markers were associated with more than one morpho-physiological trait under drought treatments. Important genomic regions were identified on chromosomes 5A and 7A. The results have clearly shown that genetic mapping is one of the feasible options to identify drought-related marker(s) in wheat mutant germplasms.
| Country or International Organization | Istanbul University, Turkey |
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Primary author
Dr
Ayse SEN
(Department of Biology, Faculty of Science, Istanbul University, 34134, Vezneciler-Fatih, Istanbul, Turkey)
Co-author
Dr
Irfan OZTURK
(Trakya Directorate of the Institute of Agricultural Research, 22100, Edirne, Turkey.)
