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15–19 May 2023
Vienna International Centre
Europe/Vienna timezone

Plasma Diagnostics of Non-thermal Atmospheric -Pressure Plasma Jet for Biomedical Application

19 May 2023, 10:50
20m
Board Room A (Vienna International Centre)

Board Room A

Vienna International Centre

Wagramer Strasse 5, Vienna, Austria A-1400
Atmospheric and Medical Plasmas Atmospheric and Medical Plasmas

Speaker

MOHAMMED Kalaf (Ministry of Science and Technology)

Description

Abstract:
In recent years, non-thermal atmospheric pressure plasma has attracted wide attention in
health care for the” processing” of medical tools and living tissues due to its many
advantages, such as non-destructive surgery, controlled, high-exactness removal of
diseased sections, high efficiency, simple systems, easy operation, non-toxic residue, and
low cost. In this work, the construction and characterization of an Atmospheric Pressure
Microwave Induced (APMI) Plasma Jet, that had been generated using microwave up to
2.4GHz for argon (Ar) and helium (He) gases and operated at low-temperature plasma
below 40 Cº for exceptional standardization protocol of this plasma source that meets
medical requirements. The plasma column has been characterized as a function of the Ar
and He flow rate. The influence of the higher gas flow rate lead to increase of the plasma
column length and reduction of the plasma jet temperature. The optical Emission
Spectroscopy (OES) method was employed to detect the active species inside the plasma
column and determine plasma parameters such as electron temperature (Te), electron
density (ne), plasma frequency (fp), Debye length (λD), and Debye (ND) number of the Ar
and He plasma jet. The plasma parameters, of the electronic excitation temperature and
density of electrons were determined by Boltzmann‟s plot method and Stark broadening
effect equation respectively. The inactivation efficiency of the APMI plasma jet is
evaluated against Gram-positive pathogenic bacteria (Staphylococcus aureus) and Gram-
negative (E. coli) with different time exposure. These samples were exposed to a plasma
column at different exposure times (5, 10, and 15 min) with an argon flow rate of 15 slm
and helium gas flow rate of 4 slm , the distance between the plasma column nozzle and
sample (bacteria) was 3 cm. The results presented that the plasma column temperature
was lower than 40 Cº, which will not cause damage to living tissues. The inactivation
efficiency is directly extended with increased exposure time and treatment with helium
plasma jets showed a higher efficiency in bacterial inhibition than argon plasma jets. The
result of the emission line spectrum showed the presence of reactive oxygen and nitrogen
species between lines 300 nm and 700 nm which formed from ambient air. These species
are the main key in the bacteria inactivation process. We confirmed that the inactivation
mechanism was unaffected by UV irradiation while the charged particles played a minor
role in the inactivation process.

Presenting Author Mohammed K. Khalaf
Presenting Author Email Address mohammedkhkh@yahoo.com
Presenting Author Affiliation Ministry of Science and Technology, Baghdad, Iraq.
Country Iraq
Presenting Author Gender Male

Primary authors

MOHAMMED Kalaf (Ministry of Science and Technology) Mr Murtdha A. Siyah (Ministry of Science and Technology)

Presentation materials

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