FAO/IAEA International Symposium on Plant Mutation Breeding and Biotechnology

IDENTIFICATION OF PROTON-BEAM INDUCED MUTATIONS IN SOYBEAN BY GENOTYPING-BY-SEQUENCING (GBS)

Not scheduled

20m

IAEA, Vienna

Oral

Speaker

Dr Jaihyunk Ryu (Chonnam National University)

Description

Mutagenesis using ionizing radiation has been widely used for the development of breeding resources. Because mutation breeding using gamma-rays has been attempted in soybean crop, studies on the effectiveness of different types of radiation are limited. This study carried out to investigate the genetic variation in the soybean chromosome induced by the proton beam irradiation. Twenty M2 plants by proton beam irradiation (10 M2 plants at 118 Gy and 10 M2 plants at 239 Gy) and two plants of original cultivar (Deapungkong) were sequenced using GBS platform. The mapping to the reference genome averaged 30 with high accuracy, and the average length of the mapped region was 125 Mb. Total of 7,453 SNPs was observed in 20 M2 plants. SNP occurred at a frequency of 1 / 36,976 bp at the proton beam irradiation dose of 118 Gy, and 1 / 32,945 bp at the 239 Gy dose. The SNPs induced by proton beam were uniformly distributed in most of the chromosomes. Gene ontology clustering analysis of mutative genes showed that the many mutations induce functional changes. The detection of the mutation loci, types, and rates in genome wide would provide the molecular characterization of mutations induced by proton beam in soybean.