FAO/IAEA International Symposium on Plant Mutation Breeding and Biotechnology

RNA-SEQ-BASED TRANSCRIPTOME ANALYSIS OF MOLECULAR VARIATIONS IN COMMON WHEAT MUTANTS DERIVED FROM GAMMA-RAYS AND EMS

Not scheduled

15m

IAEA, Vienna

Poster New challenges and technologies in plant genomics and breeding

Speaker

Dr Hongchun Xiong (Institute of Crop Sciences, Chinese Academy of Agricultural Sciences)

Description

Induced mutation has been widely used for crop improvement due to acceleration and enhancement of genetic variations. However, the molecular basis induced by physical or chemical mutagens is still largely unknown in common wheat. And mechanisms behind the phenotypic variations induced by mutagens are interesting to be clarified. Transcriptomic sequencing was performed for investigation molecular basis of induced mutation, the dwarf or salinity-resisting mechanisms in wheat mutant lines. The results showed that mutations in the EMS-induced dm20 dwarf mutant resulted in higher SNP numbers, transitions and DEGs between the wild type (WT) and dm20 compared to that in γ-rays-induced dm19 dwarf mutant, suggesting that the variation in the transcriptome of dm20 was higher than that in dm19. Most of genes encoding heat shock proteins (HSPs) showed higher expression in both dm19 and dm20 compared with WT. Furthermore, transcript levels of three genes involving auxin metabolism were significantly down-regulated in dm20. In addition, we have also screened one salinity-resisting mutant and analysis of mutated genes suggested that two mutated genes were enriched in GO term “sodium ion transport”. Moreover, “oxidation-reduction process” was the only significantly enriched GO term by the up-regulated genes between salinity-treated mutant and WT.This study supplied important information of transcriptomic sequence variation in the mutant lines and also revealed the potential mechanisms of phenotypic variations. In the dwarf mutants, the HSPs may play an important role in dwarfism and auxin may contribute to the reduction of plant height in dm20. Genes associated with sodium ion transport may directly contribute to salinity tolerance and the homeostasis of oxidation-reduction process play important roles in salt resistance in the salinity-resisting mutant.