FAO/IAEA International Symposium on Plant Mutation Breeding and Biotechnology

IMPROVEMENT OF IRANIAN RICE VARIETIES FOR SALINITY TOLERANCE THROUGH MUTATION APPROACH

Not scheduled

15m

IAEA, Vienna

Poster Mutation breeding for adaptation to climate change in seed propagated crops

Speaker

Dr Leila Bagheri (Nuclear Agriculture School, Nuclear Science and Technology Research Institute-Karaj/Iran)

Description

Rice is staple food for half of the world. Soil salinity is one of the major abiotic stresses leading to the reduction of rice yield. The objective of this research was to induce mutations for salt tolerance, high yield, early maturity and lodging resistance in local rice varieties in Iran (Tarom-mahali, Hasani and Anbarboo). Mutation breeding method by using gamma ray from 60Co was conducted during 2010-2015 rice sowing seasons. After determining of the proper dose, seeds of each variety were exposed to 200, 250, 300 Gy doses for raising M1 generations. Twenty-two thousand panicles were harvested on an individual plant basis for evaluation in the next generation. In M2 generation, seeds of all mutants and control were sown in saline soil (EC =6-8 ds/m). 430 mutants were confirmed. In M3 generation, 134 mutants were selected and inspected to confirm their genetic stability as well as to evaluate their agronomic characteristics and salinity reaction in the following generation.The evaluation of M4 mutants were done in an augment design with 9 blocks at saline field condition (EC =7.3 ds/m). Parameters of chlorophyll fluorescence were measured during vegetative growth. In flowering stage, plant height, Panicle number in hill, and fifty percent flowering were recorded. In physiological maturity stage, the superior mutant lines than the parent cultivars were harvested. By using data of paddy yield, salinity tolerant indices were calculated. The 30 selected mutant lines were evaluated in the M5 generation in a complete randomized block design with four replications. Results of yield and components yield trials and morphological and physiological assessments indicated a significant difference among mutant lines and their parents. The 17 promising mutant lines were selected, that in higher generations will be evaluated.