FAO/IAEA International Symposium on Plant Mutation Breeding and Biotechnology

ASSESSMENT OF ALLELIC VARIATION AMONG NEWLY IMPROVED RICE VARIETIES AT SALTOL REGION IN SRI LANKA

Not scheduled

15m

IAEA, Vienna

Poster New challenges and technologies in plant genomics and breeding

Speakers

Mr D.G.K.P. Wijerathna (Rice Research and Development Institute, Batalagoda, Ibbagamuwa, Sri Lanka)Dr H.M.V.G. Herath (Department of Agric. Biology. Faculty of Agriculture,University of Peradeniya)Mrs R.M.N.H. Senanayake (Rice Research and Development Institute, Batalagoda, Ibbagamuwa, Sri Lanka)Dr U.A.K.S. Udawela (Rice Research and Development Institute, Batalagoda, Ibbagamuwa, Sri Lanka)

Description

The productivity of rice is greatly affected by soil salinity which is the second most widespread soil problem after drought in rice growing areas in the world. This research was aimed to analyse Sri Lankan rice varieties grown in salinity affected areas, with the SSR markers closer to, previously identified – Saltol QTL – located on chromosome 1 and use those data in rice breeding and gene mapping studies. The research was conducted at Rice Research and Development Institute, Batalagoda, Sri Lanka. 21 improved rice varieties namely Bg379-2, Bg450, At402, Bg403, Bg406, Bg94-1, Bg352, At353, At354, Bg357, Bg358, Bg359, Bg360, At362, Ld365, Bg366, Bg369, Bg300, Bg4-91, At307, At308 and check variety Pokkali were used. Polymorphic bands obtained from SSR markers were analysed by Jaccard’s similarity coefficient following the unweight pair group method with arithmetic mean (UPGMA). DNA samples were amplified using 8 SSR markers located between 10.8- 16.4 Mb, on the Saltol QTL region of chromosome1. The eight markers used were highly polymorphic and 45 alleles were amplified with band sizes between of 100 and 300 bp. The highest number of alleles was found in RM7075 (8) followed by RM493 (6), RM562 (6), RM10748 (6), RM10582 (5), RM140 (5), RM1287 (5) and RM3412 (4) which gave the lowest number of alleles. Locus RM 562 showed the highest polymorphism information content (PIC) value (0.7831). The mean PIC value was 0.7124 and it ranged from 0.5718 to 0.7831. 0ut of 8 markers RM562 would be more useful for screening of rice germplasms. Information derived on the polymorphic SSR marker patterns obtained from tolerant and susceptible varieties near Saltol region would be useful in selecting parental lines from the tested varieties for rice breeding and gene mapping programs designed for salt tolerance.