Speakers
Mr
Christian Wagner
(BOKU-University of Natural Resources and Life Sciences Vienna, Austria)Mrs
Petra Schwarz
(BOKU-University of Natural Resources and Life Sciences Vienna, Austria)
Description
Fusarium head blight (FHB) is a severe fungal disease of wheat affecting yield and grain quality. One major QTL for FHB resistance in wheat is Qfhs.ifa-5A, which is located on the centromeric region of the wheat chromosome arm 5A. Genetic mapping of Qfhs.ifa-5A resulted in a low resolution in the Qfhs.ifa-5A region: In a near- isogenic RIL population, segregating for the Qfhs.ifa-5A resistance allele, four out of 3650 lines recombined within the pericentromeric C-5AS1-0.40 bin, yielding only a single recombination point. To further increase the map resolution a radiation induced deletion mapping approach was performed. Two gamma irradiated wheat deletion panels were created: (i) radiation selfing (RS) panel: seeds of line NIL3 carrying the Qfhs.ifa-5A resistance allele in a susceptible background were irradiated and plants thereof were selfed to obtain deletions in homozygous state and (ii) a radiation hybrid (RH) panel: irradiated pollen of the wheat line Chinese Spring (CS) was used for pollinating the CS-nullisomic5Atetrasomic5B. Overall, 5125 RS and 276 RH plants were pre-screened for deletions on 5AS. Plants having one or more markers deleted were analysed using 102 5AS-specific markers. A consensus map derived from both deletion panels results in a 380-fold map improvement (cR/cM) of the 5AS chromosome compared to the genetic mapping approach, with an average map resolution of 0.77 Mb/cR. This striking improvement in map resolution underlines the superiority of radiation induced deletion mapping over genetic linkage mapping for low recombining regions. Phenotyping the RS deletion lines can help to narrow down the QTL-interval for gene cloning.
| Country or International Organization | BOKU-University of Natural Resources and Life Sciences Vienna, Austria |
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Primary authors
Mr
Christian Wagner
(BOKU-University of Natural Resources and Life Sciences Vienna, Austria)
Mrs
Maria Buerstmayr
(BOKU-University of Natural Resources and Life Sciences Vienna, Austria)
Mrs
Petra Schwarz
(BOKU-University of Natural Resources and Life Sciences Vienna, Austria)
Co-authors
Dr
Andrea Volante
(Council for Agricultural Research and Economics, Research Centre for Cereal and Industrial Crops, Vercelli, Italy)
Dr
Barbara Steiner
(BOKU-University of Natural Resources and Life Sciences Vienna, Austria)
Dr
Delfina Barabaschi
(Council for Agricultural Research and Economics, Genomics Research Centre, Fiorenzuola d’Arda, Italy)
Dr
Giampiero Valè
(Council for Agricultural Research and Economics, Research Centre for Cereal and Industrial Crops, Vercelli, Italy)
Prof.
Hermann Buerstmayr
(BOKU-University of Natural Resources and Life Sciences Vienna, Austria)
Mr
Klaus Brugger
(BOKU-University of Natural Resources and Life Sciences Vienna, Austria)
Ms
Lisa Blazek
(BOKU-University of Natural Resources and Life Sciences Vienna, Austria)
Dr
Luigi Cattivelli
(Council for Agricultural Research and Economics (CREA), Genomics Research Centre, Fiorenzuola d’Arda, Italy)
