The cooperation with the IAEA is guided in particular by the directions and priorities defined in Kuwait’s Integrated Nuclear Security Support Plan (INSSP), whose one of the key objectives is to “establish formal governmental organization and measures for managing the national nuclear security regime” and which focusses on addressing immediate and future national nuclear security needs and priorities. The INSSP has proved to be an effective mechanism supporting a holistic strategic and graded approach to strengthening the country’s nuclear security regime and plays a key role in channeling efforts and resources towards achieving in the shortest time possible a strong and sustainable nuclear security infrastructure. Its implementation makes an important contribution to the overall security in the country through strengthening institutional, human and technical capability in various aspects of nuclear security, including control and securing of radioactive sources, national response plan, illicit trafficking and border controls. The INSSP’s scope covers all required components from the legal and regulatory framework to prevention, detection and response to prevent any unauthorized acquisition, supply, possession, use, transfer or disposal of nuclear and other radioactive material. The cooperation in the field of Nuclear Security started in 2006 where the first INSERVE mission took place followed by many meetings that led to the establishment of the INSSP in 2009. The IAEA support is extremely vital in establishing the Nuclear Security Regime .In this context, special efforts are being deployed by relevant stakeholders to strengthen further the national system for the security of radioactive materials to secure their protection against any malicious acts. The present paper outlines the cooperation with the IAEA and the significant steps undertaken and activities carried out to this effect and stresses in terms of lessons learned the importance of stakeholders’ involvement to achieve this goal.